Effects of Carbohydrates on Callus Growth and Callus Derived Plantlet Regeneration in Doritaenopsis Orchid

Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid

Embryogenic calli were induced from longitudinally bisected segments of protocorm-like bodies (PLBs) of Cymbidium Twilight Moon ‘Day Light’, a hybrid orchid, on modified Vacin and Went medium [Bot. Gaz. 110 (1949) 605] supplemented with 1-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with N-phenyl-N′-1,2,3-thiadiazol-5-yl urea (TDZ) within 1 mont...

Callus culture and regeneration.

Effects of Cultivar, Explant Treatment, and Medium Supplements on Callus Induction and Plantlet Regeneration in Perennial Ryegrass

Perennial ryegrass (Lolium perenne L.) is a widely used cool-season grass species as turf and forage. In order to use biotechnological approaches to improve the species, factors affecting tissue culture responses of mature caryopses of perennial ryegrass were studied. Callus induction and regeneration from mature caryopses of thirteen recentlyreleased turf-type perennial ryegrass cultivars were...

Promotion of callus initiation, shoot regeneration and proliferation in Lisianthus.

The effects of plant growth regulators were examined in order to optimize the callus induction, regeneration, and proliferation of lisianthus (Eustoma grandiflorum). In vitro leaves provided the explants for callus induction. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and 2,4-dichlorophen...

Effects of explant type and growth regulators on callus induction in four ecotypes of Persian shallot

Allium hirtifolium commonly known as Persian shallot is an important wild medicinal plant distributed from North West to central and South West of Iran. To establish an efficient protocol for callus induction, the effects of explant type and growth regulators on callus induction in four ecotypes of Persian shallot were evaluated. Two explants types included basal plates and young leave...